Thursday, January 5, 2012

Written in the Blood: Why Wait?

Blood. Early diagnosis is all lies between life and death. Fever, fatigue, and with the patient the pain, it's probably possible to get prompt treatment of infections. The most appropriate treatment for different types of infections, this is not always immediately clear. For diagnostic kits, blood culture may be - can grow up to the doctor, the blood in some microorganisms and yeast culture, if you are suspected of being deleted. If economic growth can be observed to investigate, determine the organization, additional tests to the essence of being, to determine the sensitivity to antibiotics. Seven days the whole process, take the worst, because ht can lead to a week.

Two years ago, Tour, at Memorial Sloan - Kettering Cancer Center to determine physician, required an increase in fungal infections, hospital mortality in cancer patients, the time for positive blood culture (antimicrobial agents and chemotherapy-related Taur British report published in January 2010, 54 (1): 184-90). In the same study, the authors emphasize a faster alternative to the cultural needs of the life-saving blood. Now, Ting - in the current work and science Wangyu Lin, Taipei University, co-workers as a market leader is the ability to take an important step.

Journal of natural communication (by Liu, 2:538, 2011 November 15) in the publication, Liu and Wang recently published an article, so that his blood samples for analysis introduced directly via spectral techniques for the detection of bacteria. This suggests that by Raman spectroscopy shows the different types of bacteria in the earlier works, which differ based on the large range of technical resources.

Conventional Raman spectroscopy has the light in the sample. Photons (particles of light) is absorbed, then the sample is released - the original and some of these two photons of different wavelengths. "Flexibility" (as opposed to wavelength contrast) of the photon distribution is called Raman scattering. Emitted photons are collected by the lens, all the photons with a wavelength close to the original, is filtered only the Raman signal. It is the wavelength of the Raman signal by changing the molecular structure information of the sample observations. As the name suggests, Raman signals in the rule for the improvement of the rough metal surface (binding) large adsorption surface Raman spectroscopy (SERS O) are used.

National Taiwan University, employees, Liu and Wang SERS substrates, antibiotic vancomycin bacterial cell wall compounds (VAN) and coatings. Recent increase in the detection of thousands of times over the range of priorities where the coating of bacteria in both the Raman signal of the test, it is important, significant SERS signal without an increase. So the writers have a bridge between the metal surface of the particle gap is estimated that the train on the metal surface, the bacterial cell wall to increase. These openings Raman scattering "hot spots" as the presence of bacteria in the vicinity of these sites may therefore represent the largest signal. Although the coating also increases the affinity of bacterial cells and reduces the affinity of the metal surface. The study also showed that resistant bacteria, so that the possibility of rapid determination of antibiotic sensitivity, the system can generate different Raman signals.

The effects of early warning systems are profound: the author is a slight modification of the protocol can adapt the detected virus particles, has been claimed. In addition, this agreement is not only a recent study; blood samples can be concentrated in the environment. Our culture, only the proportion of the bacterial species detected, not only the technical capabilities of our van, such as creating new types of bacterial genomes have been sequenced to accelerate and to identify if you do not have the rapid identification of contaminants in drinking water.
Blood.

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